Journal: Nature Immunology
Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function
doi: 10.1038/s41590-025-02381-7
Figure Lengend Snippet: ( a ) CytoF immunophenotyping of patients total CD3 + T cells, NK cells and indicated NK cell subsets. Controls 0–3 y.o. (n = 2), Controls 4–15 y.o. (n = 9), Controls 16–100 y.o. (n = 28), pediatric patients (LOH) (n = 5), adult patients (LOH) (n = 2), heterozygous individuals (n = 3), mean ± s.d. Statistical significance was assessed with multiple Mann Whitney tests adjusted for multiple testing. *p<0.05. ( b ) Assessment of T cell proliferation of patients P1, P2 and P3. (top) gating strategy, (bottom) CFSE dilution plots. ( c ) Intracellular TNF production of CD4 + and CD8 + T cell blasts in homozygous (n = 4), heterozygous (n = 2) and wildtype (n = 5) state of CBL Ub LOF variants. Line indicates the mean of the displayed datapoints. ( d,e ) Intracellular and extracellular cytokine production by patients T fh cells stimulated with CD2/CD3/CD28 under T h 0 conditions for 5 days. ( d ) Intracellular staining of the indicated cytokines. Healthy donors (n = 17), CBL-LOH patients (n = 4). Mann-Whitney testing with correction for multiple testing did not reveal significant (p< 0.05) differences between healthy donors and CBL-LOH patients. Line indicates the mean of the displayed datapoints. ( e ) Extracellular detection of cytokines by ELISA. Healthy donors (n = 6), CBL-LOH patients (n = 3). Mann-Whitney testing with correction for multiple testing did not reveal significant (p< 0.05) differences between healthy donors and CBL-LOH patients. Line indicates the mean of the displayed datapoints.
Article Snippet: 154Sm , CD3 , UCHT1 , 3154003B , Fluidigm.
Techniques: MANN-WHITNEY, Staining, Enzyme-linked Immunosorbent Assay
Bio X Cell is a verified supplier 
Bio X Cell manufactures this product 
